The Royal College of Apothecaries of the City and Kingdom of Valencia (Spanish: Real Colegio de Farmacéuticos de la Ciudad y Reino de Valencia) is a professional association of pharmacists in the Valencian region of Spain. It was founded in 1436 by King Alfonso V of Aragon in order to regulate the practice of pharmacy in the city and kingdom.

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The college was granted a royal charter in 1444, which gave it the power to set standards for the education and training of apothecaries, as well as the authority to inspect apothecary shops and ensure that they were selling safe and effective medicines.

The college offered a four-year program of study that included lectures, practical training, and an apprenticeship. The curriculum covered topics such as anatomy, botany, chemistry, and pharmacy. The first graduates of the college received their diplomas in 1447.

The Royal College of Apothecaries of the City and Kingdom of Valencia continued to operate for centuries. It was dissolved in 1840, but it was re-established in 1849. The college is still in existence today and is a leading institution for the education and training of pharmacists in Spain.